Seminar

안녕하세요 생명과학과 사무실입니다. 이번주 세미나 연사님은 성균관대학교 약학과 김충섭 교수님입니다. 김충섭 교수님은 자랑스러운 우리 학교 동문 출신으로 본교 약학대학 학부/대학원을 졸업 하셨으며, 대학원 과정 동안 다양한 생명체로부터 추출한 천연물/대사물질의 구조를 밝히고 인체질환 치료인자로서의 가능성을 규명하여 상당한 양의 논문을 게재 하였습니다. 

 이후 [인간-마이크로바이옴간의 대사적 상호작용] 연구 분야 세계적 권위자 Jason M. Crawford Lab (Yale) 에서의 포스닥 과정부터  2020년 우리 학교 약학대학 조교수로 부임하신 지금까지 마이크로바이옴에서 생성하는 새로운 대사물질들을 동정하여 그 구조와 인체 생리 및 질환에서의 기능을 규명하는 아주 흥미로운 연구를 하시고 계십니다.  이번 세미나에서는 특히 최근 Science지에 게재하신 인간 마이크로바이옴으로 부터 새롭게 동정한 Colibactin 대사 물질에 관한 Story와, 최근 연구 중이신 마이크로바이옴 대사물질 Autoinducer-3 (AI-3) 에 의한 인체면역반응 조절에 관한 Story를 강연해주실 예정 입니다.

- 오프라인 세미나 장소 : 제1과학관 3층 31317호 (기초과학연구소세미나실)

- 온라인(실시간 스트리밍) 접속링크(ZOOM)

https://us02web.zoom.us/j/3299955127?pwd=WVJkYVlkNEhqUHQxK2MvZDFwejRtUT09 (외부 사이트로 연결합니다.)
Meeting ID: 329 995 5127
Passcode: 965572

 

E. coli Metabolites that Modulate Human Physiology 

Abstract 

Escherichia coli is a well-studied model organism that has served as a transformative biological  resource, not only for unraveling central basic mechanisms of molecular genetics and cell biology, but  also for its widespread applications in molecular biology, microbiology, and biotechnology. Natural  variants of E. coli are among the first to colonize the human intestinal tract after birth and are  estimated to reside in about 90% of the population. Pathogenic strains fall within eight known  pathotypes and cause a variety of severe infections, such as meningitis, hemorrhagic colitis,  pneumonia, urinary tract infections (UTIs), hemolytic uremic syndrome, colorectal cancer, and others  with about 165,000 infections being reported annually in the United States alone. Despite the  bacterium’s expansive biomedical importance, a molecular understanding of the signaling systems  that E. coli use to regulate virulence and quorum sensing is incomplete. Here, I characterized the  structure and biosynthesis of the elusive autoinducer-3 (AI-3), providing molecular resolution to a  microbiology problem that has spanned nearly 20 years. I show that AI-3 is produced across  pathogenic, probiotic, and commensalistic strains, and the small molecule is biosynthesized by a gene  that is essential for growth. Finally, I demonstrate that AI-3 activates virulence genes in  enterohemmorhagic E. coli (EHEC). Colibactin is a gut microbiome metabolite of unknown structure that has been implicated in colorectal  cancer formation. Several studies now suggest that the tumorigenicity of colibactin derives from  interstrand cross-linking of host DNA. In this study, a combination of genetics, isotope labeling,  tandem MS, and chemical synthesis was utilized to deduce the structure of colibactin. This structural  assignment accounts for all known biosynthetic data and suggests roles for the final unaccounted  enzymes in the colibactin gene cluster. DNA cross-link degradation products derived from synthetic  and natural colibactin were indistinguishable by tandem MS analysis, thereby confirming the structure  prediction. This work reveals the structure of colibactin, which has remained incompletely defined for  over a decade.