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Identification of the Full 46 Cytochrome P450 (CYP) Complement and Mod…

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작성자 관리자 댓글 0건 조회 383회 작성일 15-06-22 02:06





The 46 cytochrome P450
(CYP) gene superfamily was identified in the marine copepod Paracyclopina nana after
searching an RNA-seq database and comparing it with other copepod CYP
gene families. To annotate the 46 Pn-CYP genes, a phylogenetic analysis
of CYP genes was performed using a Bayesian method. Pn-CYP genes
were separated into five different clans: CYP2, CYP3, CYP20, CYP26, and
mitochondrial. Among these, the principal Pn-CYP genes involved in
detoxification were identified by comparing them with those of the copepod
Tigriopus japonicus and were examined with respect to their responses to
exposure to a water-accommodated fraction (WAF) of crude oil and to the
alkylated forms of two polycyclic aromatic hydrocarbons (PAHs; phenanthrene and
fluorene). The expression of two Pn-CYP3027 genes (CYP3027F1 and
CYP3027F2) was increased in response to WAF exposure and also was
upregulated in response to the two alkylated PAHs. In particular,
Pn-CYP3027F2 showed the most notable increase in response to 80% WAF
exposure. These two responsive CYP genes (Pn-CYP3027F1 and
CYP3027F2) were also phylogenetically clustered into the same clade of
the WAF- and alkylated PAH-specific CYP genes of the copepod T.
, suggesting that these CYP genes would be those chiefly
involved in detoxification in response to WAF exposure in copepods. In this
paper, we provide information on the copepod P. nana CYP gene superfamily
and also speculate on its potential role in the detoxification of PAHs in marine
copepods. Despite the nonlethality of WAF, Pn-CYP3027F2 was rapidly and
significantly upregulated in response to WAF that may serve as a useful
biomarker of 40% or higher concentration of WAF exposure. This paper will be
helpful to better understand the molecular mechanistic events underlying the
metabolism of environmental toxicants in copepods.




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